Another quick tutorial for anyone that's interested. This is a bit hardcore but is useful foundation understanding for anyone that wants to get into microscopy.
So far I have referred to the two types of microscope being reflected and transmitted light. I am going to concentrate on transmitted light for now. That's basically looking at light passing through thin sections. But whadya know there is more than one kind of light.
Every bit of light we receive is made up of zillions of photons, little bundles of light energy. Each one has a random orientation or plane. So say you were looking at a conventional wrist watch. One photon could be arriving in your eye in the 12 o'clock 6 o'clock plane. Another in the 2 o'clock 8 o'clock plane. Yet another in the 11.30 5.30 plane etc etc a million times over in absolutely every possible plane. The eye can't tell which plane each photon is in, it doesn't matter, it's all just light.
But white light is made up of all the colours at once. Proof of this is that it can be split by a prism. Dig out a copy of Dark Side of the Moon by Pink Floyd, that's what's on the cover.
When you look at white light through a microscope this is what you get. All colours and all orientations. This is called 'Bright Field'.
There are times, though, when you might only want to get one colour of light. For this, microscopes can be fitted with colour filters. Easy.
The next bit is a bit more complicated. Sometimes you only want light in one plane of orientation. I will explain why in a minute. Say you only want light in the 12 - 6 plane. This is what polarising filters do. They have a very very fine set of lines on them that only lets those photons through that are in the right plane of orientation. Like say if you stood over a road drain with slats on it and dropped a packet of uncooked spaghetti onto it in random orientations. Only the spaghetti that was in the same orientation as the grating would get through. The rest would bounce off. This is what a polarising filter is doing, only the photons in one plane are getting through. Looking at a slide in this light is called 'Plane Polarised'.
With me so far? The polarising filter goes between the lamp and the slide so the slide is only getting polarised light through it.
What would happen if you put another polarising filter over the slide at right angles to the first one. That's right, no light can get through. Like 2 drain covers. What one lets through the other catches.
But that's not strictly true. Whatever is between them could be acting like a mini prism, in effect rotating the photons slightly. Thus a few make it through the second polariser because their plane of orientation is no longer at right angles to the second polariser. Doing this is called looking in 'Cross Polarised Light'.
OK I am going to find a picture of a rock thin section to illustrate why this is useful.