ID help please

[Tony Croft aka hamadryad]

Sorry, i think i inserted the wrong icon, twas supposed to be a smily face:001_smile:. I did not intend in anyway to be sarcy and think your efforts into doing/processing/reporting and trying to publish your own research are very very comendable and i applaude you whole-heartedly:thumbup1:

 

I should of elaborated more, what i meant to ask in full was... you state the there is no practical way to detect the myceliums in the wood or habitat. Is this set in stone due to the nature/organic structure of the said myceliums or is it that no-one has ever tried to find them in some fandangled new way?

 

well now were talking!

 

as far as i know thus far there are three ways of detecting to species level maybe four, hang on!

 

spore and fruitbody old method, requires fruit body! they can be very cagey and last raely a few days.

 

take a wood core sample and grow in agar then determine species by mycelial footprint or body if formed, mmm bit hard unless you have a lab and time.

 

or DNA again, laborius and lab confined.

 

So, there is NO way of defining ecological pressence of a fungi community via any means other than fruit body records, mad innit.

 

but thats the microbial world for you, they is teeny, and shy:thumbup:

[RobArb]

would the ecological presence of fungi of certain species (maybe especially parasitic varieties) be detectable in wood via a detectable change in wood characteristics? i suppose the problem "wood" be, how would you detect those character changes?

 

Not yet knowing different sampling/collecting/monitoring techniques (i hope i do soon know) is there a way at present to do this or does one way need "inventing"??

 

Just my mad rambling thoughts thats all:blushing:

[Tony Croft aka hamadryad]

would the ecological presence of fungi of certain species (maybe especially parasitic varieties) be detectable in wood via a detectable change in wood characteristics? i suppose the problem "wood" be, how would you detect those character changes?

 

Not yet knowing different sampling/collecting/monitoring techniques (i hope i do soon know) is there a way at present to do this or does one way need "inventing"??

 

Just my mad rambling thoughts thats all:blushing:

 

if you take a wood sample, yes, you can detect the micro vegatative hypha in the vessels/lumina, this reqiures high powered microscopy though and it wont tell you species only that mycelia are in the wood.

 

That is something we already knew, fungi are so a part of the woody structure of the tree that alan rayner believes if we vapourised all molecules of the tree standing, and all that was left after the vaporisation of the tree was any mycelial entity then the tree would be lieft as a ghostly relief due to the fact that fungi are within and upon the tree be that in a dormant or otherwise state.

 

there is no way to detect and select species via any field method other than fruit body formations. most mycelium is white, but the mycelium does have a "fingerprint" basicaly how can we provide a policy for the protection of an organism that is by its very nature not quantifiable?

 

we need a new way:thumbup1:

[RobArb]

we need a new way:thumbup1:

 

So my question again (with a bit more background info)... Is this new way researchable?

 

Or is this me just not taking no for an answer and trying to think too far out of the box??

[Tony Croft aka hamadryad]

I like out of the box, in fact i dont do in the box at all!

 

if something is new, then its researchable, NO?

 

i have no idea if thats at all possible, YET!

 

My plan is to refine translocation of wild hericiums and others using a technique im thinking of, and also defining EXACTLY what it is about thier habitats they like so much, making conservation a much simpler matter, but its all fairly irrelivent unless we find a way to asses the TRUE pressence of the species, as apposed to relying on friuting bodies, which arent always there every year, and are nomadic because they often only fruit in response to habitat loss, a panic fruit body that enables a new host to be sought out.

[Will Hinchliffe]

What living wood, say a tree? doubt it.

 

identify fungal pressence yes, maybe it can detect mycelium, but can it detect genus, let alone species?

 

THAT can only be done by cultivating a sample in a lab as far as my current knowledge tells me?

 

 

I think it works by detecting trace amounts of signature chemicals produced by fungal respiration. Due to the diverse metabolic pathways that fungi utilise to break down there substrate chemical signatures may be used to ID fungi. You would need to create a library of these chemical signatures to use as a reference. And it may be possible that a probe could be used to collect gas from inside a drilled hole. Its all mega expensive. I think the price of a portable gas mas spectrometer is very high.

 

This is all a vague memory from a lecture 7 years ago about soil fungi diversity.

 

Many species cant even be cultivated in the lab, so are currently unknown to us.

[RobArb]

what an amazing little world the kingdom of fung is!

[Tony Croft aka hamadryad]

what an amazing little world the kingdom of fung is!

 

It is indeed, a chalenge to keep one busy for not just one, but many lifetimes:001_cool: